High Pressure Effects on the Activities of Cathepsins B and D of Mackerel and Horse Mackerel Muscle

نویسندگان

  • Liliana G. FidaLGo
  • Jorge a. Saraiva
  • Santiago P. aubourG
  • Manuel vázquez
  • J. antonio TorreS
چکیده

Fidalgo L.G., Saraiva J.A., Aubourg S.P., Vázquez M., Torres J.A. (2014): High pressure effects on the activities of cathepsins B and D of mackerel and horse mackerel muscle. Czech J. Food Sci., 32: 188–193. We determined high pressure processing (HPP) effects on the activities of cathepsins B and D in the muscles of mackerel (Scomber scombrus) and horse mackerel (Trachurus trachurus). In mackerel, the cathepsin B activity decrease reached 40% at 450 MPa while in horse mackerel, low and intermediate pressures (150 and 300 MPa) caused an activity increase (30%) but at 450 MPa a decrease of up to 60%. In both species, cathepsin D activity increased after a 300 MPa treatment (up to 2-fold for mackerel and 60% for horse mackerel) and decreased on a 450 MPa treatment. The activity increase is probably due to HPP damage of lysosome releasing enzymes into the fish muscle. Based on the HPP effects on the activities of cathepsins B and D, 450 MPa may be used to reduce the proteolytic activity of cathepsin B prior to chilled or frozen storage of these fish species.

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تاریخ انتشار 2014